Order Instructions for Lab Personnel | Karius

SPECIMEN COLLECTION & PREPARATION INSTRUCTIONS (download here)

 

PREFERRED METHOD - In Plasma Preparation Tube (PPT)

1. COLLECT BLOOD IN PPT

  • 5 mL of whole blood in BD Vacutainer® PPT
  • Gently invert the tube 8-10 times after draw
  • Label the tube with collection date/time and at least 2 patient identifiers

 

2. CENTRIFUGE WITHIN 6 HOURS OF DRAW

  • 1,100 RCF (g)      | 10 mins | Ambient temperature
  • Do not transfer plasma
  • If the gel plug does not rise to separate the cells from the plasma, the tube should be re-centrifuged at a higher speed

 

3. SUBMIT A TEST REQUISITION FORM

 

4. PACKAGE SPECIMEN

  • Package the specimen and the requisition form using the supplies in the Karius Kit
  • If the specimen is unlikely to reach Karius within 96 hours of draw, specimen can be frozen directly in PPT
  • See shipping instructions below

 

 

ALTERNATIVE METHOD - In Lavender Top, K2-EDTA Tube

1. COLLECT BLOOD IN K2-EDTA TUBE

  • Gently invert the tube 8-10 times after draw

 

2. CENTRIFUGE WITHIN 24 HOURS OF DRAW

  • 1,600 RCF (g)      | 10 mins | Ambient temperature
  • For tubes less than 4 mL, refer to the tube manufacturer's instructions for centrifugation speed and time

 

3. TRANSFER PLASMA

  • Transfer a minimum of 0.7 mL      of plasma into a sterile polypropylene tube (we do not recommend pooling blood draws)
  • Take care not to disturb the cell pellet
  • Label the tube with collection date/time and at least 2 patient identifiers

 

4. GO TO STEPS 3 & 4 OF PREFERRED METHOD

SPECIMEN STABILITY (After plasma has been separated from cells)

  • Ambient temperature: 96 hours
  • -20˚C: 6 months

 

REJECTION CRITERIA

  • Plasma separated from whole blood more than 6 hours (PPT) or more than 24 hours (K2-EDTA) after draw
  • Unfrozen specimen received at Karius more than 96 hours after draw
  • Specimen with incomplete or improper separations of plasma
  • Specimen collected in an expired tube
  • Specimen with few than 2 patient identifiers on the tube

 


SHIPPING INSTRUCTIONS

 

AMBIENT TEMPERATURE (Specimen MUST arrive at Karius within 96 hours of draw)

All supplies needed for shipping at ambient temperature are included in the Karius kit.

  1. Place the specimen in the biohazard bag with an absorbent pad.
  2. Wrap the ambient gel pack around the specimen bag.
  3. Place the wrapped specimen bag in teh foil bubble pack mailer, then place the mailer in the Karius kit cardboard box. 
  4. Add the printed Karius Test Requisition Form to the Karius kit cardboard box.
  5. Place the box in a FedEx UN 3373 Category B Pak. The FedEx Pak with a preprinted return label is included in the Karius kit. Ship via FedEx First Overnight to Karius.

 

FROZEN (For specimens stored at -20˚C after plasma has been separated from cells)

  1. Place the frozen specimen in a biohazard bag with an absorbent pad.
  2. Place the specimen bag on dry ice in an appropriate container, ensuring there is enough dry ice to prevent thawing during transport.
  3. Ship via FedEx First Overnight to Karius.